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After blocking with 5% nonfat dry milk in TBS-Tween for 12 h at room temperature, blots were incubated with appropriate primary antibodies overnight at 4 C

Predrag Sikiric1, Sven Seiwerth, Rudolf Rucman, Domagoj Drmic, Mirjana Stupnisek, Antonio Kokot, Marko Sever, Ivan Zoricic, Zoran Zoricic, Lovorka Batelja, Tihomil Ziger, Kresimir Luetic, Josipa Vlainic Zarko Rasic, Martina Lovric Bencic ~ World J Gastroenterol

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Thats why many peptide drugs are delivered subcutaneously (say, through an injector pen or insulin pump) and why it took so long to develop a pill version of semaglutide

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