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Frozen cortical samples (the weights of which were determined beforehand) were homogenized in 5% 5-sulfosalicylic acid (SSA), and the mixtures were centrifuged at 8000 g for 10 min at 4 C

Protocol 2: conservative approach for higher-risk researchers Who this is for: Older adults, those with borderline high resting heart rate, anyone on cardiovascular medications, history of anxiety about cardiac symptoms

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Neighborhood environment and DNA methylation: implications for cardiovascular disease risk