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2A), which were also visualized using immunofluorescence staining (Supplementary Figure 4b)

Before transducing the Brunello lentivirial library, Cas9 nuclease was stably expressed in FIXgla-Fas/HEK293 reporter cells by lentiviral transduction with lentiCas9-Blast 64 and subsequently selected using 5 g/ml blasticidin and expanded for 7 days