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2b, Extended Data Fig

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This process involves a phosphorylation cascade of key kinases [89], which mechanistically corresponds to the phosphoproteomic alterations observed in uterine fibroids [87]

Tissues were homogenized in RIPA buffer (Tris (50 mM), NaCl (150 mM), Triton X-100 (1%), disodium EDTA (1 mM), SDS (0.1%), sodium deoxycholate (0.5%)) containing protease and phosphatase inhibitors (S8830, SigmaFast, Thermo Scientific) followed with mild sonication (5 min, cycle: 30 s on/off