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Chen Sci Rep 2025;15:18063

However, many clinicians and some researchers feel there is a large grey zone between 160-500 pmol/L, which in some patients could represent functional deficiency on a cellular level

Recommended Dosing Protocols Common bodybuilding and recovery-focused protocols include: Subcutaneous: 200500 mcg once or twice daily, near the injury site or in abdominal fat Intramuscular: 200300 mcg directly into the affected muscle, rotating sites Oral: 250500 mcg dissolved in water once daily, though bioavailability may vary Many protocols begin at the lower end, around 200 mcg, during the first week to assess tolerance

[1] Unlike fat-soluble vitamins that accumulate in body tissues, water-soluble vitamins are generally excreted through urine when consumed in excess