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When pairing this protocol with peptide therapy, timing aligns with endogenous hormone peaks

Data acquisition for ABPP Peptides were analysed by LCMS/MS using an Evosep One (Evosep) coupled with a timsTOF HT (Bruker) equipped with either an 8 cm 150 m, 1.5 m analytical column (Evosep, 60SPD, ABPP samples) or a 15 cm 150 m, 1.9 m analytical column (Evosep, 30SPD, SK-MEL-103 total proteome samples)

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