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Immunoblot analysis of protein expression Cells were lysed with 0.1% Triton X-100 in TBS (10 mM TrisHCl and 150 mM NaCl, pH 7.4) in the presence of protease inhibitors (complete, 04693132001, Roche)

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Our results clearly demonstrated that HFD consumption was associated with hepatic activation of both JNK and caspase-3 (Fig