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Brains were removed, postfixed overnight, and allowed to sink in 2.5% paraformaldehyde supplemented with 30% sucrose in PBS solution

Historically, clinical endpoints such as response rates, progression-free survival, and overall survival have dominated clinical research

This is to fully characterize the full efficacy of GSHee but also to take into account recent evidence from elegant in situ brainstem studies showing that morphine affects key areas of the respiratory network in a dose-dependent manner 122

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