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Cells were then incubated with a primary antibody for 12 h at room temperature, followed by the corresponding fluorophore-conjugated secondary antibody (Alexa Fluor) for 45 min and DAPI staining (1 g ml 1 DAPI

doi: 10.1186/s12935024-032218 176 ImranMNadeemMSaeedFImranAKhanMRKhanMAet al