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A clear step-by-step research guide explaining how lyophilized peptides are reconstituted, why freeze-dried peptides offer better preparation control than pre-mixed liquid products, and how stability, sterile handling, and COA verification

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Protein concentration was determined using a BCA kit (Thermo Scientific), after which samples were diluted to 1 mg ml 1 in 5% SDS and 50 mM TEAB