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The differences between groups were analyzed using one-way ANOVA followed with Fishers LSD test (Statistica 9, Statsoft, Tulsa, OK, USA)

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Subcutaneous injection creates a depot that releases into capillary beds at a rate determined by local blood flow and interstitial pressure

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Dosing Protocol Standard Research Protocol Dose: 300mcg per day subcutaneous Timing: Fasted ideally 3060 minutes before eating or exercise Injection site: Lower abdomen or thigh (standard subQ technique) Frequency: Daily, or 5 days on / 2 days off Cycle length: 812 weeks, then 46 week break Reconstitution AOD-9604 dissolves readily in bacteriostatic water (BAC water) no acetic acid required, unlike some hydrophobic peptides