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The practical sequence: stabilize first, test second, correct nutrient and biochemical imbalances third, optimize medication selection while the patient is becoming more stable, and only then consider a slow prescriber-guided taper if the need for medication has clearly diminished

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SupersmartTM2 ZAPA3G SYBR Green qPCR Mix (Zsgentech, Tianjin, China) and cobas z 480 Automatic Fluorescence Quantitative PCR analyzer (Roche, Germany) were used for RT-qPCR, and the reaction conditions were set as follows: at 95 for 5 min, 40 cycles at 95 for 10 s, at 60 for 20 s, and at 70 for 10 s